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Cortisol ELISA assay kits are designed for the quantitative measurement of the glucocorticoid hormone cortisol in human serum and plasma. Cortisol is responsible for a variety of metabolic processes in the body including bone formation, gluconeogenesis, lipolysis, and immune responses. Researchers and health professionals can use cortisol ELISA kits to study metabolic processes and disorders.
Cortisol is a glucocorticoid produced by the adrenal cortex and is involved in blood pressure regulation, immune function, vascular tone, and glucose metabolism. It is also involved in bone metabolism and glucose counter-regulation. Cortisol is a primary glucocorticoid that affects glucose homeostasis and insulin secretion. Excess cortisol levels are associated with a variety of diseases including diabetes, Alzheimer's disease, and prostate cancer.
The Cortisol ELISA is the most common method for assessing cortisol levels. Cortisol can be measured in urine, plasma, saliva, and dried fecal extracts. It is widely used and reproducible and results are obtained in less than three hours. Absorbance at 405 nm is measured. The assay is highly reliable and has been validated for use in various applications.
Cortisol ELISA assays are competitive immunoassays for measuring cortisol in serum or plasma. The assay is not intended for diagnostic use, but for research purposes. If you're conducting your own research, the Abcam Cortisol ELISA is an excellent choice for assessing cortisol levels. This competitive assay has been developed for the quantitative measurement of cortisol.
Cortisol (Saliva) ELISA kit uses a specific antibody for cortisol to assess the presence of the hormone in biological samples. Cortisol is present in the blood as free steroid and is bound to an enzyme called corticosteroid-binding globulin. The result is an accurate measurement of the levels of Cortisol in the blood and saliva.
After detetion, there maybe some residual substances on the ELISA plate. In order to reduce the errors caused by the residues, an ELISA washer is needed. This medical device has been widely used in the cleaning of ELISA plates in hospitals, blood stations, health and epidemic prevention stations, reagent factories and research laboratories.
For the assay, samples containing particulates must be centrifuged to remove these particles. Moderately hemolyzed samples should not be used. The assay kit comes with a cortisol standard, which must be used to read the samples. Then, a cortisol-peroxidase conjugate is added to each well. The resulting color is measured at 450 nm and is inversely proportional to the amount of cortisol in the sample.